The Baker Company Blog

Hypoxia-Inducible Factors Paper Now Available!

Posted by Beth Hildreth on Tue, Mar 21, 2017 @ 02:35 PM

Baker Ruskinn co-sponsored the Nantes Oct '16 conference focused on “the role of hypoxia under physiological conditions as well as in cancer”. 

The main objective of this conference was to bring together a large group of scientists from different spheres of hypoxia. Download the paper: 'Update on hypoxia-inducible factors and hydroxylases in oxygen regulatory pathways: from physiology to therapeautics' to review recent advances in:  genomics, physiology, musculoskeletal, stem cells, microenvironment and cancer, and oxidative stress. 

View Baker's solutions designed for Hypoxia including our Invivo2 Physiological Cell Culture Workstations

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Tags: Hypoxia Cell Culture

Biosafety Cabinet Selection Use and Lifespan Annex E Now Available

Posted by Beth Hildreth on Fri, Mar 10, 2017 @ 12:00 PM

 

Published February 2017
NSF/ANSI 49 - 2016 Annex E "Biosafety Cabinet Selection, Installation, Use, Lifespan and Decommissioning" is now available.  This free document from NSF International, Ann Arbor MI, is the United States normative reference for biosafety cabinet (BSC) practices. 

NSF/ANSI 49 - 2016 Annex E "Biosafety Cabinet Selection, Installation, Use, Lifespan and Decommissioning”

 Topics covered Include:

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Tags: Biosafety Cabinet

Baker Ruskinn at King's College London - Replicating Physiological Oxygen Levels

Posted by Beth Hildreth on Thu, Mar 09, 2017 @ 11:59 AM

On February 23rd the Inaugural Physiological Oxygen Workshop and Educational Initiative which Baker helped to pioneer, was held at King's College London.  The workshop highlighted the importance of replicating physiological oxygen levels for standard in vitro culture models with cardiovascular cells.  Hands on product training with Baker and Baker Ruskinn products included: SterilGARD, InvivO2, HypoxyCOOL and SCI-tive Dual.

Workshop attendees were all graduate students of KCL from groups with an interest in Physiological Oxygen; everyone left motivated to think again about their method of cell culture.  In addition, 45 attendees listened to a keynote lecture by Nick Forsyth of Keele University with perspectives and insights on the impact of Physiological Oxygen.

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Tags: Hypoxia Chamber, Hypoxia Workstation, physiological, in vivo, cell culture

Toward a More Accurate Description of "Low-Oxygen" Cell Culture Work

Posted by Dr. Kara Held on Fri, Jul 29, 2016 @ 04:00 PM

It’s funny how things can get labeled with certain words that don't completely make sense. Misnomers are all over the place. Jellyfish aren’t fish. Rhode Island isn’t an island. Koala bears are marsupials. The West Indies are nowhere near India.  Chinese checkers didn’t originate in China. The last two examples were named based on the current knowledge that was later found to be incorrect.

This is happening in the field of science formerly known as hypoxia. Hypoxia literally means “less oxygen.” "Less" is a relative term, and therefore, a comparison between at least two situations must exist. It has become standard to compare "hypoxic" environments to our primary surroundings; i.e., anything less than atmospheric oxygen (21% O2) has been referred to as "hypoxia." When working with plants or whole animals, this seems appropriate. They normally breathe 21% atmospheric oxygen, so anything less would be considered hypoxic. But what about cells? Organs? Tissues? Are they normally exposed to 21% oxygen? The answer is no. 

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Can your microbiology lab afford not to use an anaerobic workstation?

Posted by Cheri Gaudet on Thu, Jun 02, 2016 @ 04:23 PM


Most microbiology lab managers and PIs purchase jars to run experiments, rather than purchase an anaerobic workstation. The up-front cost to use jars is low compared with a workstation, and since the jars can be reused for years, it is common to assume that they are cheaper over time as well. But have you considered the cost of consumables?

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Tags: Anaerobic Workstation

Clean Air and Containment for Your Compounding Pharmacy

Posted by Dr. Kara Held on Wed, May 11, 2016 @ 01:02 PM

Clean_Air_and_Containment_Decision_Map_Pharmacy_thumb.jpgWith all the regulations that need to be followed for pharmacy compounding, it can be difficult to determine what kind of clean air and containment equipment (such as biosafety cabinets and clean benches) you need for your facility. USP <795>, <797>, and <800>, as well as NIOSH, ASHP, OSHA and CETA - which guideline tells me which cabinet to work in?

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Tags: Pharmacy, Hazardous Drug Compounding, Pharmaceutical Compounding, Sterile Drug Compounding

Will vibration destroy my cell culture?

Posted by Dr. Kara Held on Mon, Apr 18, 2016 @ 02:12 PM

A hot new concept coming through the world of cell culture is the notion that the current “gold standard” method of culturing cells is good, but could be better. The use of incubators and separate biosafety cabinets for media changes can introduce a whole host of variability to your culturing conditions. Being transported between warm, humid, CO2-primed air and ambient air can put significant stress on your cells.  This is especially true when working with hypoxia.  A hypoxic shock, or a few-hour treatment of a hypoxic environment, may help increase your desired results (e.g., upregulation of hypoxia-induced genes), but this short-term treatment is not replicating the natural environment that the cells should be exposed to.  In the body, cells are mostly in a hypoxic (lower than ambient air, or normoxic) environment.

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Tags: Hypoxia Cell Culture

USP <800> Engineering Controls Q&A

Posted by Dr. Kara Held on Fri, Feb 19, 2016 @ 10:06 AM

Thank you to all who joined us for our recent webinar on USP <800> and its impact to engineering controls! If you didn't get a chance to attend, the webinar is available on-demand here. A special thanks to all who asked such good questions, both before and during the webinar. We received more questions than we could answer in a one-hour webinar, so here for the benefit of all are our responses to your questions.

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Tags: Hazardous Drug Compounding

Baker Ruskinn Solutions Now Available Through SciPro

Posted by Cheri Gaudet on Thu, Feb 18, 2016 @ 10:36 AM

Baker Ruskinn has exciting news for researchers conducting anaerobic and low-oxygen cell culture in North America. Our controlled-oxygen workstations and media conditioning solutions are now available from SciPro, a distributor of scientific instruments and specialty accessories, including a full range of solutions for monitoring and controlling oxygen in cell culture. The partnership was announced today in a press release on Baker's website.

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Tags: Hypoxia Cell Culture

Improving Stem Cell Yield with Media Conditioning

Posted by Cheri Gaudet on Mon, Feb 08, 2016 @ 09:00 AM

In this video, Prof. Nicholas Forsyth, Associate Director of the Institute of Science and Technology at Keele University in the UK, discusses how the use of a media conditioning system helped his team improve stem cell yield by creating a physiologically-relevant environment for cells directly in culture media.

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Tags: Media Conditioning